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easysep human cord blood cd34 positive selection kit iii  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc easysep human cord blood cd34 positive selection kit iii
    Easysep Human Cord Blood Cd34 Positive Selection Kit Iii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+cd34+selection+kit/easysep+human+t+cell+isolation+kit/pm40632850-144-6-15
    Average 90 stars, based on 1 article reviews
    easysep human cord blood cd34 positive selection kit iii - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: IMPDH inhibition activates TLR‐VCAM1 pathway and suppresses the development of MLL‐fusion leukemia
    Article Snippet: Retrovirus transduction to the leukemia cells was performed using Retronectin (Takara Bio Inc, Otsu, Shiga, Japan). .. Human CB CD34 + cells were isolated using an EasySep CD34 selection kit (STEMCELL Technologies) or a CD34 MicroBead Kit (Miltenyi Biotec). .. The CB CD34 + cells, CB cells transduced with MLL‐AF9, MLL‐ENL, or RUNX1‐ETO, and primary human AML cells were cultured in Iscove's modified Dulbecco's media (IMDM) containing 20% BIT9500 (STEMCELL Technologies) or StemSpan SFEM II medium (#ST‐09655, STEMCELL Technologies) containing 1% penicillin–streptomycin together with 10 ng/ml rhSCF (#255‐SC, R&D Systems), 10 ng/ml rhTPO (#288‐TP, R&D Systems), 10 ng/ml rmFlt‐3 ligand (#427‐FL, R&D Systems), 10 ng/ml rhIL‐3 (#203‐IL, R&D Systems) and 10 ng/ml rhIL‐6 (#206‐IL, R&D Systems; Goyama et al , ).

    Article Title: Reprogramming of Embryonic Human Fibroblasts into Fetal Hematopoietic Progenitors by Fusion with Human Fetal Liver CD34 + Cells
    Article Snippet: .. Mononuclear cells were isolated using gradient centrifugation in HISTOPAQUE-1077 (Sigma-Aldrich, St. Louis, USA). hFL CD34+ cells were isolated using EasySep CD34 Selection Kit (StemCell Technologies, Vancouver, Canada) according to the manufacturer's instructions. ..

    Article Title: Alternative RUNX1 Promoter Regulation by Wnt/β-Catenin Signaling in Leukemia Cells and Human Hematopoietic Progenitors.
    Article Snippet: MAT IAS A. MEDINA, GIORGIA D. UGARTE, MACARENA F. VARGAS, MIGUEL E. AVILA, DAVID NECU~ NIR, ALVARO A. ELORZA, SORAYA E. GUTI ERREZ, AND GIANCARLO V. DE FERRARI* Faculty of Biological Sciences and Faculty of Medicine, Center for Biomedical Research, Universidad Andres Bello, Santiago, Chile Millennium Institute on Immunology and Immunotherapy, Santiago, Chile Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Universidad de Concepci on, Concepci on, Chile

    Article Title: Method for inducing hemoblast differentiation
    Article Snippet: <1-3> Differentiation into Basophilic Erythroblasts The erythroblasts obtained in example <1-2> were suspension-cultured in plasma-free and serum-free Stemline (Sigma, U.S.) containing 50 ng/ml of stem cell factor (SCF; R&D Systems, U.S.) and 2 IU/ml of erythropoietin (EPO; Calbiochem, U.S.) for 2 days, to be differentiated and proliferated into basophilic erythroblasts which are one kind of erythroid progenitor cells. .. CD34 positive cells were isolated from the collected cord blood using EasySep CD34 selection kit (StemCell Technologies, Canada) by the immunomagnetic microbead selection method (Partington et al., J. Immunol. .. <1-4> Differentiation into Polychromatic Erythroblasts The erythroblasts obtained in example <1-2> were suspension-cultured in plasma-free and serum-free Stemline (Sigma, U.S.) containing 50 ng/ml of stem cell factor (SCF; R&D Systems, U.S.) and 2 IU/ml of erythropoietin (EPO; Calbiochem, U.S.) for 4 days, to be differentiated and proliferated into polychromatic erythroblasts which are one kind of erythroid progenitor cells.

    Selection:

    Article Title: IMPDH inhibition activates TLR‐VCAM1 pathway and suppresses the development of MLL‐fusion leukemia
    Article Snippet: Retrovirus transduction to the leukemia cells was performed using Retronectin (Takara Bio Inc, Otsu, Shiga, Japan). .. Human CB CD34 + cells were isolated using an EasySep CD34 selection kit (STEMCELL Technologies) or a CD34 MicroBead Kit (Miltenyi Biotec). .. The CB CD34 + cells, CB cells transduced with MLL‐AF9, MLL‐ENL, or RUNX1‐ETO, and primary human AML cells were cultured in Iscove's modified Dulbecco's media (IMDM) containing 20% BIT9500 (STEMCELL Technologies) or StemSpan SFEM II medium (#ST‐09655, STEMCELL Technologies) containing 1% penicillin–streptomycin together with 10 ng/ml rhSCF (#255‐SC, R&D Systems), 10 ng/ml rhTPO (#288‐TP, R&D Systems), 10 ng/ml rmFlt‐3 ligand (#427‐FL, R&D Systems), 10 ng/ml rhIL‐3 (#203‐IL, R&D Systems) and 10 ng/ml rhIL‐6 (#206‐IL, R&D Systems; Goyama et al , ).

    Article Title: Flow cytometric characterization of freshly isolated and culture expanded human synovial cell populations in patients with chronic arthritis
    Article Snippet: .. Partial enrichment for CD34-positive fraction was performed with an EasySep CD34 selection kit using the clone QBend10 (Stem Cell Technologies, Grenoble, France). ..

    Article Title: Protease-activated receptor-1 inhibits proliferation but enhances leukemia stem cell activity in acute myeloid leukemia
    Article Snippet: .. CD34 + cells were separated using EasySep CD34 Selection Kit (STEMCELL Technologies, Vancouver, BC, Canada). ..

    Article Title: Reprogramming of Embryonic Human Fibroblasts into Fetal Hematopoietic Progenitors by Fusion with Human Fetal Liver CD34 + Cells
    Article Snippet: .. Mononuclear cells were isolated using gradient centrifugation in HISTOPAQUE-1077 (Sigma-Aldrich, St. Louis, USA). hFL CD34+ cells were isolated using EasySep CD34 Selection Kit (StemCell Technologies, Vancouver, Canada) according to the manufacturer's instructions. ..

    Article Title: Alternative RUNX1 Promoter Regulation by Wnt/β-Catenin Signaling in Leukemia Cells and Human Hematopoietic Progenitors.
    Article Snippet: MAT IAS A. MEDINA, GIORGIA D. UGARTE, MACARENA F. VARGAS, MIGUEL E. AVILA, DAVID NECU~ NIR, ALVARO A. ELORZA, SORAYA E. GUTI ERREZ, AND GIANCARLO V. DE FERRARI* Faculty of Biological Sciences and Faculty of Medicine, Center for Biomedical Research, Universidad Andres Bello, Santiago, Chile Millennium Institute on Immunology and Immunotherapy, Santiago, Chile Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Universidad de Concepci on, Concepci on, Chile

    Article Title: Method for inducing hemoblast differentiation
    Article Snippet: <1-3> Differentiation into Basophilic Erythroblasts The erythroblasts obtained in example <1-2> were suspension-cultured in plasma-free and serum-free Stemline (Sigma, U.S.) containing 50 ng/ml of stem cell factor (SCF; R&D Systems, U.S.) and 2 IU/ml of erythropoietin (EPO; Calbiochem, U.S.) for 2 days, to be differentiated and proliferated into basophilic erythroblasts which are one kind of erythroid progenitor cells. .. CD34 positive cells were isolated from the collected cord blood using EasySep CD34 selection kit (StemCell Technologies, Canada) by the immunomagnetic microbead selection method (Partington et al., J. Immunol. .. <1-4> Differentiation into Polychromatic Erythroblasts The erythroblasts obtained in example <1-2> were suspension-cultured in plasma-free and serum-free Stemline (Sigma, U.S.) containing 50 ng/ml of stem cell factor (SCF; R&D Systems, U.S.) and 2 IU/ml of erythropoietin (EPO; Calbiochem, U.S.) for 4 days, to be differentiated and proliferated into polychromatic erythroblasts which are one kind of erythroid progenitor cells.

    Article Title: Alternative translation initiation generates the N-terminal truncated form of RUNX1 that retains hematopoietic activity.
    Article Snippet: Transcription factor RUNX1 plays a crucial role in hematopoiesis, and its activity is tightly regulated at both transcriptional and post-translational levels.. However, translational control of RUNX1 expression has not been fully understood.. In this study, we demonstrated that RUNX1b mRNA is translated from two alternative initiation sites (Met-1 and Met-25), giving full-length RUNX1b and a shorter protein lacking the first 24 amino acids (RUNX1 N24).

    Gradient Centrifugation:

    Article Title: Reprogramming of Embryonic Human Fibroblasts into Fetal Hematopoietic Progenitors by Fusion with Human Fetal Liver CD34 + Cells
    Article Snippet: .. Mononuclear cells were isolated using gradient centrifugation in HISTOPAQUE-1077 (Sigma-Aldrich, St. Louis, USA). hFL CD34+ cells were isolated using EasySep CD34 Selection Kit (StemCell Technologies, Vancouver, Canada) according to the manufacturer's instructions. ..

    Article Title: Alternative RUNX1 Promoter Regulation by Wnt/β-Catenin Signaling in Leukemia Cells and Human Hematopoietic Progenitors.
    Article Snippet: MAT IAS A. MEDINA, GIORGIA D. UGARTE, MACARENA F. VARGAS, MIGUEL E. AVILA, DAVID NECU~ NIR, ALVARO A. ELORZA, SORAYA E. GUTI ERREZ, AND GIANCARLO V. DE FERRARI* Faculty of Biological Sciences and Faculty of Medicine, Center for Biomedical Research, Universidad Andres Bello, Santiago, Chile Millennium Institute on Immunology and Immunotherapy, Santiago, Chile Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Universidad de Concepci on, Concepci on, Chile



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    NSG-Quad mice support human hematopoietic cell engraftment and multilineage immune cell development (A) Percentage of human CD45 + (hCD45 + ) cells of total CD45 + cells (mouse and human) in the blood at 8 weeks and 10–15 weeks post-engraftment with human-cord-blood-derived <t>CD34</t> + cells. (B) Human immune cell composition in the blood of NSG ( n = 33), NSG-Quad ( n = 34), and MISTRG-6 mice ( n = 32) 8 weeks post-engraftment and in the blood of NSG ( n = 29), NSG-Quad ( n = 25), and MISTRG-6 mice ( n = 16) 10–15 weeks post-engraftment. B cells (CD3 − CD56 − CD33 − CD20 + ), myeloid cells (CD3 − CD56 − CD20 − CD33 + ), NK cells (CD3 − CD56 + ), CD8 + T cells (CD3 + CD4 − CD8 + ), and CD4 + T cells (CD3 + CD8 − CD4 + ). (C) Percentage of human immune cell subsets in the blood 8 weeks post-engraftment (data from B). (D) Percentage of human immune cell subsets in the blood 10–15 weeks post-engraftment (data from B). Data are shown as mean ± SEM. p values were calculated using one-way or two-way ANOVA (A) with Tukey’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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    NSG-Quad mice support human hematopoietic cell engraftment and multilineage immune cell development (A) Percentage of human CD45 + (hCD45 + ) cells of total CD45 + cells (mouse and human) in the blood at 8 weeks and 10–15 weeks post-engraftment with human-cord-blood-derived <t>CD34</t> + cells. (B) Human immune cell composition in the blood of NSG ( n = 33), NSG-Quad ( n = 34), and MISTRG-6 mice ( n = 32) 8 weeks post-engraftment and in the blood of NSG ( n = 29), NSG-Quad ( n = 25), and MISTRG-6 mice ( n = 16) 10–15 weeks post-engraftment. B cells (CD3 − CD56 − CD33 − CD20 + ), myeloid cells (CD3 − CD56 − CD20 − CD33 + ), NK cells (CD3 − CD56 + ), CD8 + T cells (CD3 + CD4 − CD8 + ), and CD4 + T cells (CD3 + CD8 − CD4 + ). (C) Percentage of human immune cell subsets in the blood 8 weeks post-engraftment (data from B). (D) Percentage of human immune cell subsets in the blood 10–15 weeks post-engraftment (data from B). Data are shown as mean ± SEM. p values were calculated using one-way or two-way ANOVA (A) with Tukey’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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    NSG-Quad mice support human hematopoietic cell engraftment and multilineage immune cell development (A) Percentage of human CD45 + (hCD45 + ) cells of total CD45 + cells (mouse and human) in the blood at 8 weeks and 10–15 weeks post-engraftment with human-cord-blood-derived <t>CD34</t> + cells. (B) Human immune cell composition in the blood of NSG ( n = 33), NSG-Quad ( n = 34), and MISTRG-6 mice ( n = 32) 8 weeks post-engraftment and in the blood of NSG ( n = 29), NSG-Quad ( n = 25), and MISTRG-6 mice ( n = 16) 10–15 weeks post-engraftment. B cells (CD3 − CD56 − CD33 − CD20 + ), myeloid cells (CD3 − CD56 − CD20 − CD33 + ), NK cells (CD3 − CD56 + ), CD8 + T cells (CD3 + CD4 − CD8 + ), and CD4 + T cells (CD3 + CD8 − CD4 + ). (C) Percentage of human immune cell subsets in the blood 8 weeks post-engraftment (data from B). (D) Percentage of human immune cell subsets in the blood 10–15 weeks post-engraftment (data from B). Data are shown as mean ± SEM. p values were calculated using one-way or two-way ANOVA (A) with Tukey’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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    NSG-Quad mice support human hematopoietic cell engraftment and multilineage immune cell development (A) Percentage of human CD45 + (hCD45 + ) cells of total CD45 + cells (mouse and human) in the blood at 8 weeks and 10–15 weeks post-engraftment with human-cord-blood-derived <t>CD34</t> + cells. (B) Human immune cell composition in the blood of NSG ( n = 33), NSG-Quad ( n = 34), and MISTRG-6 mice ( n = 32) 8 weeks post-engraftment and in the blood of NSG ( n = 29), NSG-Quad ( n = 25), and MISTRG-6 mice ( n = 16) 10–15 weeks post-engraftment. B cells (CD3 − CD56 − CD33 − CD20 + ), myeloid cells (CD3 − CD56 − CD20 − CD33 + ), NK cells (CD3 − CD56 + ), CD8 + T cells (CD3 + CD4 − CD8 + ), and CD4 + T cells (CD3 + CD8 − CD4 + ). (C) Percentage of human immune cell subsets in the blood 8 weeks post-engraftment (data from B). (D) Percentage of human immune cell subsets in the blood 10–15 weeks post-engraftment (data from B). Data are shown as mean ± SEM. p values were calculated using one-way or two-way ANOVA (A) with Tukey’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
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    Image Search Results


    NSG-Quad mice support human hematopoietic cell engraftment and multilineage immune cell development (A) Percentage of human CD45 + (hCD45 + ) cells of total CD45 + cells (mouse and human) in the blood at 8 weeks and 10–15 weeks post-engraftment with human-cord-blood-derived CD34 + cells. (B) Human immune cell composition in the blood of NSG ( n = 33), NSG-Quad ( n = 34), and MISTRG-6 mice ( n = 32) 8 weeks post-engraftment and in the blood of NSG ( n = 29), NSG-Quad ( n = 25), and MISTRG-6 mice ( n = 16) 10–15 weeks post-engraftment. B cells (CD3 − CD56 − CD33 − CD20 + ), myeloid cells (CD3 − CD56 − CD20 − CD33 + ), NK cells (CD3 − CD56 + ), CD8 + T cells (CD3 + CD4 − CD8 + ), and CD4 + T cells (CD3 + CD8 − CD4 + ). (C) Percentage of human immune cell subsets in the blood 8 weeks post-engraftment (data from B). (D) Percentage of human immune cell subsets in the blood 10–15 weeks post-engraftment (data from B). Data are shown as mean ± SEM. p values were calculated using one-way or two-way ANOVA (A) with Tukey’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Comparison of NSG-Quad and MISTRG-6 humanized mice for modeling circulating and tumor-infiltrating human myeloid cells

    doi: 10.1016/j.omtm.2025.101487

    Figure Lengend Snippet: NSG-Quad mice support human hematopoietic cell engraftment and multilineage immune cell development (A) Percentage of human CD45 + (hCD45 + ) cells of total CD45 + cells (mouse and human) in the blood at 8 weeks and 10–15 weeks post-engraftment with human-cord-blood-derived CD34 + cells. (B) Human immune cell composition in the blood of NSG ( n = 33), NSG-Quad ( n = 34), and MISTRG-6 mice ( n = 32) 8 weeks post-engraftment and in the blood of NSG ( n = 29), NSG-Quad ( n = 25), and MISTRG-6 mice ( n = 16) 10–15 weeks post-engraftment. B cells (CD3 − CD56 − CD33 − CD20 + ), myeloid cells (CD3 − CD56 − CD20 − CD33 + ), NK cells (CD3 − CD56 + ), CD8 + T cells (CD3 + CD4 − CD8 + ), and CD4 + T cells (CD3 + CD8 − CD4 + ). (C) Percentage of human immune cell subsets in the blood 8 weeks post-engraftment (data from B). (D) Percentage of human immune cell subsets in the blood 10–15 weeks post-engraftment (data from B). Data are shown as mean ± SEM. p values were calculated using one-way or two-way ANOVA (A) with Tukey’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Article Snippet: Human CD34 + HSPCs were isolated from cord blood using an EasySep Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies, #17896).

    Techniques: Derivative Assay, Comparison

    NSG-Quad mice support the development of human myeloid cells (A) Percentage of human CD45 + (hCD45 + ) cells of total CD45 + cells (mouse and human) in the blood of NSG-Quad +/− mice (heterozygous M-CSF, n = 24) and NSG-Quad +/+ mice (homozygous M-CSF, n = 9) at 10–15 weeks post-engraftment with human-cord-blood-derived CD34 + cells. (B) Human immune cell composition in the blood of NSG-Quad +/− mice ( n = 24) and NSG-Quad +/+ mice ( n = 9) 10–15 weeks post-engraftment. (C) Percentage of human immune cell subsets in the blood of NSG-Quad +/− and NSG-Quad +/+ mice 10–15 weeks post-engraftment (data from B). (D) Percentage of human CD45 + (hCD45 + ) cells of total CD45 + cells (mouse and human) in the blood at 8 weeks and 10–15 weeks post-engraftment with human-cord-blood-derived CD34 + cells. (E) Human immune cell composition in the blood of NSGS ( n = 11) and NSG-Quad mice ( n = 25) 10–15 weeks post-engraftment. (F) Percentage of human immune cell subsets in the blood of NSGS and NSG-Quad mice 10–15 weeks post-engraftment (data from E). Data are shown as mean ± SEM. p values were calculated using two-tailed, unpaired Student’s t test (A), two-way ANOVA with Tukey’s multiple comparison test (D) and two-tailed, unpaired Mann-Whitney U test (C, F). ∗ p < 0.05, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Comparison of NSG-Quad and MISTRG-6 humanized mice for modeling circulating and tumor-infiltrating human myeloid cells

    doi: 10.1016/j.omtm.2025.101487

    Figure Lengend Snippet: NSG-Quad mice support the development of human myeloid cells (A) Percentage of human CD45 + (hCD45 + ) cells of total CD45 + cells (mouse and human) in the blood of NSG-Quad +/− mice (heterozygous M-CSF, n = 24) and NSG-Quad +/+ mice (homozygous M-CSF, n = 9) at 10–15 weeks post-engraftment with human-cord-blood-derived CD34 + cells. (B) Human immune cell composition in the blood of NSG-Quad +/− mice ( n = 24) and NSG-Quad +/+ mice ( n = 9) 10–15 weeks post-engraftment. (C) Percentage of human immune cell subsets in the blood of NSG-Quad +/− and NSG-Quad +/+ mice 10–15 weeks post-engraftment (data from B). (D) Percentage of human CD45 + (hCD45 + ) cells of total CD45 + cells (mouse and human) in the blood at 8 weeks and 10–15 weeks post-engraftment with human-cord-blood-derived CD34 + cells. (E) Human immune cell composition in the blood of NSGS ( n = 11) and NSG-Quad mice ( n = 25) 10–15 weeks post-engraftment. (F) Percentage of human immune cell subsets in the blood of NSGS and NSG-Quad mice 10–15 weeks post-engraftment (data from E). Data are shown as mean ± SEM. p values were calculated using two-tailed, unpaired Student’s t test (A), two-way ANOVA with Tukey’s multiple comparison test (D) and two-tailed, unpaired Mann-Whitney U test (C, F). ∗ p < 0.05, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Article Snippet: Human CD34 + HSPCs were isolated from cord blood using an EasySep Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies, #17896).

    Techniques: Derivative Assay, Two Tailed Test, Comparison, MANN-WHITNEY

    NSG-Quad mice promote the development of tumor-infiltrating human macrophages (A) Tumor growth curves in NSG ( n = 9), NSG-Quad ( n = 12), and MISTRG-6 mice ( n = 15) engrafted with SW480 CRC cells and in NSG ( n = 10), NSG-Quad ( n = 4), and MISTRG-6 mice ( n = 14) engrafted with HCT116 CRC cells. (B) Frequency of hCD45 + cells in the tumor of NSG, NSG-Quad, and MISTRG-6 humanized mice engrafted with SW480 or HCT116 CRC cells (10–15 weeks post-engraftment with human-cord-blood-derived CD34 + cells; end of experiment). (C) Human immune cell composition in the blood of NSG, NSG-Quad, and MISTRG-6 humanized mice engrafted with SW480 or HCT116 CRC cells (10–15 weeks post-engraftment with human-cord-blood-derived CD34 + cells; end of experiment; data from G). (D) Human immune cell composition in the tumor xenografts of NSG ( n = 9, SW480; n = 10, HCT116), NSG-Quad ( n = 10, SW480; n = 3, HCT116), and MISTRG-6 mice ( n = 15, SW480; n = 12, HCT116). (E) Representative IHC pictures show SW480 CRC-infiltrating human CD68 + macrophages in NSG, NSG-Quad, and MISTRG-6 humanized mice. Scale bar: 50 μm. (F) Frequency of human CD68 + macrophages in CRC xenografts of NSG ( n = 11), NSG-Quad ( n = 7), and MISTRG-6 mice ( n = 15) analyzed by IHC. (G) Frequency of CD86 + (M1-like) and CD163 + (M2-like) human CD14 + monocytes in SW480 and HCT116 CRC xenografts of NSG-Quad ( n = 13) and MISTRG-6 mice ( n = 17). (H) Composition of the human CD33 + CD66b − myeloid cell population based on the expression of CD14, CD16, and HLA-DR in SW480 CRC xenografts of NSG-Quad ( n = 8) and MISTRG-6 ( n = 3) mice. Data are shown as mean ± SEM. p values were calculated using one-way or two-way ANOVA (G) with Tukey’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Comparison of NSG-Quad and MISTRG-6 humanized mice for modeling circulating and tumor-infiltrating human myeloid cells

    doi: 10.1016/j.omtm.2025.101487

    Figure Lengend Snippet: NSG-Quad mice promote the development of tumor-infiltrating human macrophages (A) Tumor growth curves in NSG ( n = 9), NSG-Quad ( n = 12), and MISTRG-6 mice ( n = 15) engrafted with SW480 CRC cells and in NSG ( n = 10), NSG-Quad ( n = 4), and MISTRG-6 mice ( n = 14) engrafted with HCT116 CRC cells. (B) Frequency of hCD45 + cells in the tumor of NSG, NSG-Quad, and MISTRG-6 humanized mice engrafted with SW480 or HCT116 CRC cells (10–15 weeks post-engraftment with human-cord-blood-derived CD34 + cells; end of experiment). (C) Human immune cell composition in the blood of NSG, NSG-Quad, and MISTRG-6 humanized mice engrafted with SW480 or HCT116 CRC cells (10–15 weeks post-engraftment with human-cord-blood-derived CD34 + cells; end of experiment; data from G). (D) Human immune cell composition in the tumor xenografts of NSG ( n = 9, SW480; n = 10, HCT116), NSG-Quad ( n = 10, SW480; n = 3, HCT116), and MISTRG-6 mice ( n = 15, SW480; n = 12, HCT116). (E) Representative IHC pictures show SW480 CRC-infiltrating human CD68 + macrophages in NSG, NSG-Quad, and MISTRG-6 humanized mice. Scale bar: 50 μm. (F) Frequency of human CD68 + macrophages in CRC xenografts of NSG ( n = 11), NSG-Quad ( n = 7), and MISTRG-6 mice ( n = 15) analyzed by IHC. (G) Frequency of CD86 + (M1-like) and CD163 + (M2-like) human CD14 + monocytes in SW480 and HCT116 CRC xenografts of NSG-Quad ( n = 13) and MISTRG-6 mice ( n = 17). (H) Composition of the human CD33 + CD66b − myeloid cell population based on the expression of CD14, CD16, and HLA-DR in SW480 CRC xenografts of NSG-Quad ( n = 8) and MISTRG-6 ( n = 3) mice. Data are shown as mean ± SEM. p values were calculated using one-way or two-way ANOVA (G) with Tukey’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

    Article Snippet: Human CD34 + HSPCs were isolated from cord blood using an EasySep Human Cord Blood CD34 Positive Selection Kit II (StemCell Technologies, #17896).

    Techniques: Derivative Assay, Expressing, Comparison